EcoRI RFLP linked to the humanmybgene
نویسندگان
چکیده
منابع مشابه
Human gastrin (GAS) RFLP recognised by digestion with BamHI or EcoRI.
SOURCE AUD DESCRIPTION OF CLONE: The human gastrin gene cDNA insert in clone pHG529 (1) is 463 bp in length. This excludes the poly (dC) tail* and includes 8 nucleotides of 5' untranslated region, the entire coding region of 303 nucleotides, a 3 1 untranslated region of 102 nucleotides and a poly (A) track of 50 residues.
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Steady-state parameters governing cleavage of pBR322 DNA by EcoRI endonuclease are highly sensitive to ionic environment, with K(m) and k(cat) increasing 1,000-fold and 15-fold, respectively, when ionic strength is increased from 0.059 to 0.23 M. By contrast, pre-steady-state analysis has shown that recognition, as well as first and second strand cleavage events that occur once the enzyme has a...
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chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...
15 صفحه اولRFLPs in human X-linked PGK1: a new probe for the PstI RFLP demonstrates strong linkage disequilibrium with the BgII RFLP.
INTRODUCTION: Several RFLFs have been described in the human phosphoglycerate kinase 1 (PGK1) gene in Xql3. Whereas the PstI polymorphism as detected with PGK1 cDNA (Hutz et al.) was complicated by multiple X-linked and autosomal constant bands, only two bands are seen with a fourth-intron genomic probe. We have compared this PstI RFLP to the 5' Bgll RFLP detected by pSPT-PGK (Vogelstein et al....
متن کاملSequences spanning the EcoRI substrate site.
Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.4.1928